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• Product name Anti-Bad抗体[Y208] (DyLight? 488)
  参阅全部 Bad 一抗
• Description

  兔单克隆抗体[Y208] to Bad (DyLight? 488)

• Conjugation DyLight? 488. Ex: 493nm, Em: 518nm
• Specificity ab139836 does not cross-react with other Bcl-2 members.
• Tested applications Flow Cytmore details
• Species reactivity

  Reacts with:Mouse, Rat, Human

• Immunogen

  Synthetic peptide corresponding to residues at the N terminal of Human Bad (UniProt ID: Q92934).

• Positive control
  • HeLa cells.
• General notes Produced under U.S. Patent No. 5,675,063.

Properties

• Form Liquid
• Storage instructions Store at +4°C.
• Storage buffer pH: 7.20
  Preservative: 0.02% Sodium azide
  Constituents: 99% PBS, 0.05% BSA

• Concentration 100 μl at 0.2 mg/ml

• Purity Immunogen affinity purified
• Clonality Monoclonal
• Clone number Y208
• Isotype IgG
• Research Areas
  • Cell Biology
  • 
    
  • Apoptosis
  • 
    
  • Intracellular
  • 
    
  • Bcl2 Family
  • Cancer
  • 
    
  • Invasion/microenvironment
  • 
    
  • Apoptosis
  • 
    
  • Bcl 2 family
  • Metabolism
  • 
    
  • Pathways and Processes
  • 
    
  • Metabolism processes
  • 
    
  • Apoptosis

Applications

Our Abpromise guarantee covers the use ofab139836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• Function Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
• Tissue specificity Expressed in a wide variety of tissues.
• Sequence similarities Belongs to the Bcl-2 family.
• Domain Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
• Post-translational
  modifications Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
• Cellular localization Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
• Information by UniProt

• Database links

  • Entrez Gene: 572 Human
  • Entrez Gene: 12015 Mouse
  • Entrez Gene: 64639 Rat
  • Omim: 603167 Human
  • SwissProt: Q92934 Human
  • SwissProt: Q61337 Mouse
  • SwissProt: O35147 Rat
  • Unigene: 370254 Human

  see all

• Alternative names

  • BAD antibody
  • BAD_HUMAN antibody
  • BBC 2 antibody

  see all

Anti-Bad antibody [Y208] (DyLight? 488) images

• Flow Cytometry - Anti-Bad antibody [Y208] (DyLight? 488) - N-terminal (ab139836)

  Flow cytometric analysis of HeLa cells labelling Bad with ab139836 at 1/50 dilution (red) compared to a negative control antibody (green).